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type 45ti rotor  (Danaher Inc)


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    Structured Review

    Danaher Inc type 45ti rotor
    Type 45ti Rotor, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/type+45ti+rotor/Type+45Ti+Fixed+Angle+Rotor/pmc12755392-242-19-22
    Average 96 stars, based on 1597 article reviews
    type 45ti rotor - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Lysis:

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: .. Membranes and cell debris were pelleted by ultracentrifugation at ~95,000g in a Type 45Ti rotor (Beckman-Coulter) for 30 minutes, resuspended in lysis buffer using a 1:5 weight (cell pellet) to volume ratio, flash frozen in liquid nitrogen and stored at -80oC. .. For purification, membranes were solubilized with 1% DDM (Anatrace) and 0.1% CHS (Anatrace) for 1 hour at 4oC while stirring.

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: .. Membranes were pelleted by ultracentrifugation at ~95,000g in a Type 45Ti rotor (Beckman-Coulter) for 30 minutes, resuspended in lysis buffer, flash frozen in liquid nitrogen, and stored at -80oC. .. Membranes were solubilized with 1% DDM (Anatrace) for 1 hour.

    Centrifugation:

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: .. Membranes from the resulting supernatant were then pelleted by centrifugation at ~158,000g in a Type 45Ti rotor (BeckmanCoulter) for 1 hour. ..

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: ALG enzymes were solubilized by adding 1% DDM (Anatrace) and 0.1% cholesteryl hemisuccinate (CHS, Anatrace) for 1 hour stirring. .. Cellular debris was removed by centrifugation at ~125,000g in a Type 45Ti rotor (Beckman-Coulter) for 30 minutes. .. Imidazole pH 8.0 was added to the supernatant to a final concentration of 25 mM before loading onto a 5 mL column of Superflow NTA resin (Qiagen) equilibrated in ALG1 buffer containing 20 mM HEPES, pH 7.5; 150 mM NaCl; 5 mM β-mercaptoethanol; 0.03% AR TI CL E IN P RE SS ARTICLE IN PRESS (w/v) DDM; 0.006% (w/v) CHS, and 25 mM imidazole pH 8.0.

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: .. The lysate was then incubated at 60oC for 30 minutes before cellular debris and aggerated protein was removed by centrifugation at ~125,000g in a Type 45Ti rotor (Beckman-Coulter) for 30 minutes. ..

    Article Title: p62 sorts Lupus La and selected microRNAs into breast cancer-derived exosomes
    Article Snippet: .. EV-depleted medium was produced by centrifugation at 186,000 × g (40,000 RPM) for 24 h at 4°C using a Type 45Ti rotor (Beckman Coulter). ..

    Incubation:

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: .. The lysate was then incubated at 60oC for 30 minutes before cellular debris and aggerated protein was removed by centrifugation at ~125,000g in a Type 45Ti rotor (Beckman-Coulter) for 30 minutes. ..

    Isolation:

    Article Title: GLYCO-BUILD: an enzymatic pipeline for the synthesis of peptides carrying eukaryotic N-glycans.
    Article Snippet: .. Isolated membranes were extracted with 1% (w/v) DDM (Anatrace) for 1 hour before insoluble components were pelleted at ~125,000g in a Type 45Ti rotor (Beckman-Coulter) for 30 minutes. ..

    Produced:

    Article Title: p62 sorts Lupus La and selected microRNAs into breast cancer-derived exosomes
    Article Snippet: .. EV-depleted medium was produced by centrifugation at 186,000 × g (40,000 RPM) for 24 h at 4°C using a Type 45Ti rotor (Beckman Coulter). ..



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